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Blue Sky Biotech, Inc.

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Blue Sky Biotech, Inc.



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Protein Sciences and Protein Purification
Price: N/A

Protein Sciences and Protein Purification

FPLC: IEC, IMA, SEC, AC, Buffer-exchange, etc.
Microsome preparation
Tissue/organ extraction and purification
Antibody purification
Development of multi-step protocols
Activity validation (where applicable)
QC supported by MASS Spec service
QC by Western Blot and SDS PAGE

Purified recombinant protein is indispensable for drug discovery. In the early stages of drug discovery, recombinant protein is used for target validation, enzyme characterization, high-throughput screening (HTS), and structural study. In order to understand a disease target, pharmaceutical scientists must study its interaction with substrates. For this purpose, researchers need active protein free of impurities which could interfere with their study of its mechanism. Purity and activity of the recombinant protein are essential: a pure, active enzyme gives a clean signal and reproducible results.

Blue Sky employees have worked in the Protein Science department of a major pharmaceutical company. This group and others like it serve almost as "core facilities" in that they provide made-to-order proteins for enzyme characterization, structural study, and HTS. Our experience in this environment gives us a unique understanding of the requirements for quality, purity, and activity of recombinant proteins. Customers of Blue Sky may rest assured that all purified proteins provided by this firm will meet the most exacting standards.

Protein expression, purification, and characterization are very precise, difficult disciplines. We at Blue Sky have the expertise to generate the high-quality protein reagents which you need.


Our most common protein services include the following:

Generation of expression strains in E. coli and yeast ( S. cerevisiae, P. pastoris, S. pombe) systems; expression of recombinant protein in E. coli and yeast systems
Generation of recombinant baculoviruses and protein expression in insect cells with he baculovirus system
Protein purification by affinity, ion-exchange, and size-exclusion chromatography
Protein analysis by gel electrophoresis and mass spectrometry


Other, less-common services include the following:

Expression of recombinant protein in mammalian cells by plasmid- and retrovirus-based systems
Protein purification by reverse-phase HPLC and other methods of chromatography
Protein analysis by 2-D electrophoresis, N-terminal sequencing, and other methods

If you need a service which you do not see on this list, please contact us. In most cases, we will be able to perform it for you!

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