PR.com: Business Directory, Press Releases, Jobs, Products, Services, Articles
 
Businesses Articles Press Releases Follow @PRcom
Press Release Pricing | News by Category | News by Country | News by US Region | Recent News | PR.com News on Your Site
 

Press Releases

 
AnaSpec, EGT Group

Press Release


Receive press releases from AnaSpec, EGT Group: By Email RSS Feeds:

AnaSpec Offers SensoLyteŽ 520 TACE Assay Kit


San Jose, CA, August 24, 2011 --(PR.com)-- While fluorimetric assay kits for the detection and inhibitor screening of Tumor Necrosis Factor alpha (TNF-α) Converting Enzyme (TACE) currently exist, none of these have been able to take full advantage of the performance provided by long wavelength FRET technologies.

AnaSpec’s SensoLyte® 520 TACE Assay Kit offers fluorescence detection at the industry’s longest wavelengths. With superior fluorescence quantum yield and longer emission wavelength, this kit shows less autofluorescence interference from test compounds and cellular components, and provides better assay sensitivity than comparable kits using shorter-wavelength FRET substrates.

The SensoLyte® 520 TACE Activity Assay Kit uses a FRET peptide substrate for the continuous measurement of enzyme activity. The FRET pair is composed of 5-FAM (5-carboxyfluorescein) and QXL™ 520, one of AnaSpec’s proprietary fluorescence quenchers. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. The kit can be used to detect the activity of TACE or screen for TACE inhibitors.

Tumor Necrosis Factor alpha (TNF-α) Converting Enzyme (TACE), also called ADAM17 or α-Secretase belongs to the ADAM (A Disintegrin and Metalloprotease) family of proteins involved in myogenesis, neurogenesis, fertilization through the ectodomain shedding of cell surface proteins.1 TACE, the protease responsible for the generation of mature TNF-α, plays an important role in acute and chronic inflammation. Since TNF-α is a crucial mediator in the inflammatory process, considerable efforts have been made in the research and development of anti-TNF-α agents, for the purpose of reducing the severity of inflammatory responses in disease states.2,3 The inhibition of TACE by a pharmacological agent may represent an alternative approach to modulate the effect of TNF-α.4 TACE is also responsible for the proteolytic cleavage of amyloid precursor protein, L-selectin, transforming growth factor-α.1,5,6

References:
1. Peschon, JJ. et al. Science 282, 1281 (1998).
2. Feldman, M. et al. Transplant Proc. 30, 4126 (1998).
3. Siegel, SA. et al. Cytokine 7, 15 (1995)
4. Levin, JI. et al. Bioorg. Med. Chem. Lett. 13, 2799 (2003).
5. Buxbaum, JD. et al. J. Biol. Chem. 273, 27765 (1998).
6. Smalley, DM. and KJ. Ley, Cell. Mol. Med. 9, 255 (2005).

For more information, please visit https://www.anaspec.com/products/promotions.asp?id=87&col=1&row=1

AnaSpec, EGT Group
AnaSpec, EGT Group is a leading provider of integrated proteomics and genomics solutionsTM for worldwide life science research. AnaSpec offers expertise in peptides, detection reagents, antibodies, assay kits, amino acids, oligonucleotides, and qPCRs. AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening and drug discovery. AnaSpec also provides premier custom services including peptide synthesis, antibody production, assay development, fluorescent dyes, and oligonucleotide synthesis. AnaSpec is certified to ISO 9001:2008.

For more information visit www.anaspec.com

###
Contact Information
AnaSpec, Inc.
Tiffany Kao
510-791-9560
Contact
www.anaspec.com

Click here to view the list of recent Press Releases from AnaSpec, EGT Group
Promote Your Business