AnaSpec Highlights Its Proprietary HiLyte Fluorâ„¢ Dye Collection

San Jose, CA, January 17, 2012 --( AnaSpec, EGT Group’s proprietary HiLyte Fluor™ dyes are a series of excellent fluorescent labeling dyes with fluorescence emissions that span the full visible and near infrared spectrum. The hydrophilic property of most of the HiLyte Fluor™ dyes makes conjugations to protein easier, minimizing the use of organic solvents. The resulting conjugates are relatively resistant to aggregation and precipitation during storage. HiLyte Fluor™ dyes also have better labeling performance than the classic fluorescent labeling dyes such as FITC and rhodamines.

AnaSpec HiLyte Fluor™ dyes are available in a variety of reactive forms. HiLyte Fluor™ conjugates exhibit more intense fluorescence than other spectrally similar conjugates of classic fluorescent dyes such as FITC, TAMRA and ROX under similar excitations. They are more photostable than the classic fluorescent dyes such as FITC, Cy3™ and Cy5™. Also, absorption spectra of HiLyte Fluor™ dyes match the principal output wavelengths of common excitation sources such as 488 nm, 555 nm, 633 nm and 647 nm. They are highly fluorescent over a broad pH range with little pH sensitivity.

Well-separated spectra, HiLyte Fluor™ dyes are enabling many options for multicolor detection of multiple parameters simultaneously in flow cytometry analysis. HiLyte Fluor™ dye-antibody conjugates are validated for Western blot, Immunofluorescence staining and flow cytometry. HiLyte Fluor™ dyes and AnaSpec’s proprietary quenchers QXL™ have been used as fluorophore and quencher pairs for fluorescence “resonance energy transfer (FRET). Can be used to make tandem conjugates with fluorescent proteins, such as PE, APC.

FITC, the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, has its limitations, such as severe photobleaching in microscopy imaging and pH sensitivity. However, protein conjugates prepared with HiLyte Fluor™ 488 dyes (Ex/Em=497 nm/525 nm) are far more superior to conjugates of FITC (Figure 2). HiLyte Fluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Extinction coefficient is 70,000 M-1cm-1, with a quantum yield of 0.91. Additionally, the fluorescence of HiLyte Fluor™ 488 is not affected by pH (working pH range is 4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9.

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AnaSpec, Inc.
Tiffany Kao