Miami, FL, November 15, 2014 --(PR.com
)-- Source Molecular Corporation will be offering a Droplet Digital Polymerase Chain Reaction (PCR) service on large projects requiring identification of the source of fecal pollution in water samples. Droplet Digital PCR is an advanced technology that provides absolute quantification of the target DNA, allowing water managers to know not only the source of fecal pollution but also exactly how much fecal bacteria is in the water.
Source Molecular worked with scientists from the Southern California Coastal Water Research Project (SCCWRP), who are conducting an inter-laboratory research study on the use of Droplet Digital PCR to monitor environmental contaminants in water. Since Source Molecular was one of the laboratories that provided support to the researchers, it is very familiar with implementing this technology. A paper related to that study will be published in the near future.
SCCWRP and Bio-Rad Laboratories, the manufacturer of the Droplet Digital™ PCR equipment, released a video last month about this technology. In the five-minute video, SCCWRP scientists Dr. John Griffith and Dr. Yiping Cao discussed the importance of the application of the digital PCR technology on water quality monitoring projects. The video can be accessed here -- https://www.youtube.com/watch?v=S5SvaPxwXiU
According to Dr. Cao, they are interested in Droplet Digital PCR technology because it: (1) provides absolute quantification without the need for external standards, (2) is more robust against inhibition, and (3) allows multiplexing, which means it can measure multiple organisms at once. Dr. Cao developed an assay that measures both Enterococcus bacteria and a human fecal indicator organism to measure of performance of Droplet Digital PCR technology.
“Our hope with this new Droplet Digital PCR assay is that this becomes a valuable tool for monitoring agencies so they can reduce the vulnerability in their results and lower the chances of having false negatives,” Dr. Cao said.
About the Company
Source Molecular Corporation is a private commercial laboratory founded in 2002 with a mission to fill the void between source identification research and real-world implementation of the technology. Throughout the years, the Source Molecular microbiology laboratory has analyzed samples for hundreds of industrial plants, watershed management groups, state/federal government agencies, universities and engineering firms making it the leader in the Microbial Source Tracking (MST) industry. By providing commercial MST testing services, Source Molecular assists clients with recreational water quality monitoring and supports Total Maximum Daily Load (TMDL) development and implementation.