Austin, TX, January 10, 2015 --(PR.com
)-- The NEXTflex™ 18S ITS Amplicon-Seq Kit simplifies the preparation of multiplexed amplicon libraries spanning the ITS1 and ITS2 hypervariable regions of eukaryotic 18S ribosomal RNA (rRNA) genes to permit the fast and cost-effective characterization of uncultured fungal and micro-eukaryotic organisms.
Up to 384 multiplexing barcodes are available with this kit, to greatly reduce the cost of 18S ITS NGS studies on the Illumina® MiSeq platform. Libraries prepared using this kit are compatible with regular Illumina sequencing primers.
NEXTflex 18S ITS amplicon processing involves two main steps: an initial PCR amplification using customized PCR primers that target the 18S ITS domains, and a subsequent PCR amplification that integrates relevant flow cell binding domains and unique 12 base pair sample indices. The number of cleanup steps is limited to ensure maximum recovery of amplicons for downstream sequencing.
The NEXTflex 18S ITS Amplicon-Seq Kit is the latest addition to Bioo Scientific’s line of library prep kits for Illumina-compatible metagenomics analysis, which includes the NEXTflex™ 16S V1 – V3 and 16S V4 Amplicon-Seq Kits.
About Bioo Scientific Corporation
Bioo Scientific is an Austin, TX based biotechnology company that provides innovative solutions to the life science industry. Bioo Scientific offers a complete portfolio of products that increase the sensitivity, flexibility and speed of next-generation sequencing.
Bioo Scientific’s products are used around the world and can be purchased directly or through our global distribution network with markets in more than 100 countries. For more information about Bioo Scientific’s NGS product line, visit our website at www.BiooScientific.com.