Business Directory, Press Releases, Jobs, Products, Services, Articles
Businesses Articles Press Releases Follow @PRcom
Press Release Pricing | News by Category | News by Country | News by US Region | Recent News | News on Your Site
MoBiTec GmbH

Company Overview

Contact Info & Offices

Press Releases


MoBiTec GmbH

Press Release

Receive press releases from MoBiTec GmbH: By Email RSS Feeds:

MoBiTec Launches SpeedBlot (His) – A Strikingly Fast and Easy-To-Use Western Blot Stain for His-Tagged Proteins

Instant detection of poly-histidine tagged proteins on nitrocellulose membrane with SpeedBlot (His) is easy and does not require relevant hands-on time. Even 0.1 pmol detection limits can be achieved with this highly sensitive detection reagent. While common Western Blot protocols require 6 to 21 hours, staining with SpeedBlot (His) is completed after just about 90 minutes.

Göttingen, Germany, June 12, 2014 --( Western Blot is widely used in research to separate and identify proteins. In this technique a mixture of proteins is separated based on molecular weight through gel electrophoresis. The content of the gel is then transferred to a membrane producing a band for each protein. The protein of interest is then detected by means of a specific antibody.

Poly-histidine tags are often used for affinity purification of poly-histidine-tagged recombinant proteins expressed in Escherichia coli, Bacillus megaterium, Bacillus subtilis, and other prokaryotic expression systems. A poly-histidine tag is an amino acid motif in proteins that consists of multiple histidine (His) residues, mostly located at the N- or C-terminus of the protein. In order to facilitate the detection of successfully expressed his-tagged proteins the usage of anti-poly-histidine antibodies is a prevalent technique.

However, the common protocol for the antibody-based detection of the protein of interest is a laborious and time-consuming undertaking.

As time is money and to make life easier MoBiTec introduces SpeedBlot (His), a ready-to-use reagent for the detection of poly-histidine tagged proteins in blot assays:

SpeedBlot (His) is equally suitable for Western Blot assays and Dot or Slot Blot assays. The detection reagent is made of an optimized anti-histidine antibody gold nanoparticle conjugate and is reactive against C- and N-terminal tags. The protein of interest is specifically stained in pink directly on the blot.

As Easy as Ponceau Red but as Specific as ECL Antibody!


• Very fast protocol; results in 30-90 min
• Specific staining of His-tagged proteins
• Sensitive to sub-picomol amounts of protein
• Simple incubation, no buffer exchange, etc.
• Economic, no readout equipment required
• Visually detectable result
• Quantitative and permanent signal
• Works with C-/ N-terminal tags

The product is readily available. For further details please see:

Time is money! (Benjamin Franklin)
Contact Information
MoBiTec GmbH
Arne Schulz

Click here to view the company profile of MoBiTec GmbH
Click here to view the list of recent Press Releases from MoBiTec GmbH
Promote Your Business