Goettingen, Germany, September 23, 2016 --(PR.com
)-- Lentivirus is an enveloped, single-stranded RNA virus from the Retroviridae family capable of infecting both dividing and non-dividing cells. In conjunction with an efficient host-genome integration mechanism, this capability has led to recombinant lentivirus becoming a central gene delivery tool for robust and stable transgene expression in target cells.
To produce lentivirus, cells are transfected with packaging plasmids encoding the required gag, pol, and rev structural and regulatory genes as well as a transfer vector encoding the gene of interest (GOI). Replication incompetence is achieved by expressing the minimal virus components on separate plasmids and incorporating the self in-activating (SIN) elements through a major deletion of the 3' long terminal repeat (LTR) within the transfer vector. Essential components are combined with the VSV envelope protein G for a broad virus tropism and increased stability during purification procedures. The lentivirus particles are secreted into the culture medium where they are collected, filtered and frozen into aliquots for subsequent transduction into target cells.
HEK 293T cells are commonly used for lentivirus production due to high transfection efficiencies and protein expression levels; however, the lentivirus platform is often limited by insufficient viral titers requiring concentration before use. With TransIT®-Lenti, manufactured by Mirus Bio,US, greater than twofold higher functional titers are achieved over competing transfection reagents.
TransduceIT™ Reagent, an auxiliary compound (not included), is an aqueous solution of hexadimethrine bromide, a cationic polymer, that is shown to greatly enhance retroviral transduction and transgene expression in both dividing and quiescent mammalian cells. Final TransduceIT™ Reagent concentrations of 5-10 μg/ml are appropriate for most transductions, although the optimal concentration for each cell type should be empirically determined.
• High Performance – Provide up to eight-fold higher functional titers
• Simple Protocol – No media change required, single harvest
• Animal Origin Free – Regulatory friendly
Note: TransIT®-Lenti Transfection Reagent is only recommended for adherent cell transfections (e.g., HEK 293T/17) and is not recommended for lentivirus generation in suspension cell types. For highest lentivirus titers in suspension 293 cell types, we recommend using TransIT-PRO® Transfection Reagent.
· Ingenio® Electroporation Solution and Kits
· Label IT® Plasmid Delivery Controls
· Label IT® RNAi Delivery Controls
· Label IT® Tracker™ Intracellular Nucleic Acid Localization Kits
· MiraCLEAN® Endotoxin Removal Kits
· TransIT-X2® Dynamic Delivery System
· TransIT®-2020 Transfection Reagent
· TransIT®-LT1 Transfection Reagent
· TransIT® Cell Line Specific Transfection Reagents and Kits
· TransIT-siQUEST® Transfection Reagent
· TransIT-TKO® Transfection Reagent
· TransduceIT™ Reagent
For details on the above mentioned products, visit www.mobitec.com
About MoBiTec GmbH
MoBiTec GmbH (Goettingen, Germany) is a privately held company, founded in 1987, that offers research tools for molecular and cell biology. Products include DNA vectors for cloning and expression, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, products for genomics, proteomics, lipidomics, and glycomics research, numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity chromatography products, as well as general laboratory equipment.
In parallel to its own product lines, MoBiTec distributes products from international companies in Germany. MoBiTec products are distributed worldwide, in Germany from their home office, in other countries by distributors.